CELL CULTURE LIQUID/GAS-PHASE CHAMBER DEVICES AND RELATED METHODS, AND EXEMPLARY USE FOR MEASURING ACCUMULATION OF GAS-PHASE NITRIC OXIDE IN DIFFERENTIATED CULTURES OF NORMAL AND CYSTIC FIBROSIS AIRWAY EPITHELIAL CELLS

摘要

Embodiments disclosed herein include cell culture liquid/gas-phase chamber devices and related methods that allow measurement of gas-phase components and their production rate as well as substances/mediators released into media by cultured cells in response to various stimuli. In embodiments disclosed herein, these chamber devices are cell culture liquid/gas-phase chamber devices. The gas and liquid phase compartments are separated by the cell culture liquid/gas-phase chamber devices so that there is no, or minimal, physico-chemical interaction between the liquid and gas phase compartments. As an example, these cell culture liquid/gas-phase chamber devices can be used to study the effects of various pharmacologic agents or other interventions on healthy or diseased cell metabolism by assessing a release of metabolic products in liquid (culture media) and the headspace under standardized conditions. The reusable cell culture chamber devices allow for studying cultured cell responses under a short or prolonged incubation time.

主权项

1. A liquid/gas-phase chamber device for producing gas-phase and liquid-phase components released by cultured cells in response to stimuli, comprising:
a chamber body forming an internal chamber; an air-liquid partition disposed in the internal chamber configured to interactively separate the internal chamber into a liquid compartment and a gas compartment; at least one transwell disposed through the air-liquid partition, and at least one transwell configured to support at least one cell culture; the at least one transwell comprising a first end disposed in the liquid compartment of the chamber body to capture a release of metabolic products from the cells of the at least one cell culture in liquid disposed in the liquid compartment; and the at least one transwell further comprising a second end disposed in the gas compartment of the internal chamber to capture a release of gas by the cells of the at least one cell culture.