HYDROLASE ENZYME SUBSTRATES AND USES THEREOF

摘要

The present invention provides novel methods for determining the presence or amount of a hydrolytic enzyme in a sample, based on novel substrates for the enzymes, and also provides compositions and methods that provide highly sensitive assay methods for such hydrolytic enzymes.

主权项

1. A hydrolytic enzyme substrate, which is a compound of formula (I): wherein: A is an aromatic or heteroaromatic group, a 1-alkene or a 1-alkyne, each of which is optionally substituted; each R is independently H or an optionally substituted C1-C4 alkyl or aryl; n is an integer from 1-4; and X is a group comprising a substrate moiety,
wherein the substrate moiety comprises a recognition component of a substrate for the hydrolytic enzyme, and wherein the activity of said hydrolytic enzyme is capable of hydrolyzing said compound of formula (I) to form compounds (II) and (III):