发明名称 |
DETECTION OF PROTEIN TO PROTEIN INTERACTIONS |
摘要 |
The present invention relates to methods and kits for detecting the interaction between a first membrane protein and a second protein in mammalian cells as well as to identify molecules that can disrupt protein to protein interactions. The invention relies on the functional reconstitution of an active human ubiquitin by two inactive fragments upon the interaction of two proteins attached through a linker to the inactive fragments. The reconstituted ubiquitin is then cleaved by human ubiquitin proteases resulting in the release of an artificial transcription factor, which in turn activates a reporter gene transcription. Activation of the reporter gene is indicative of the interaction between the two proteins. |
申请公布号 |
WO2014197986(A1) |
申请公布日期 |
2014.12.18 |
申请号 |
WO2014CA50539 |
申请日期 |
2014.06.10 |
申请人 |
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO |
发明人 |
STAGLJAR, IGOR;PETSCHNIGG, JULIA;GROISMAN, BELLA |
分类号 |
C12Q1/68;C07K14/47;C07K14/71;C12Q1/02;G01N33/567;G01N33/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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