PLANT GENES INVOLVED IN NITRATE UPTAKE AND METABOLISM

摘要

The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.

主权项

1. A nucleic acid construct comprising:
a nucleic acid molecule that is modulated by nitrogen in corn; a 5′ DNA promoter sequence; and a 3′ terminator sequence, wherein the nucleic acid molecule, the DNA promoter sequence, and the terminator sequence are operatively coupled to permit transcription of the nucleic acid molecule, wherein the nucleic acid molecule either: (a) encodes a polypeptide having an amino acid sequence of SEQ ID NO:45; or (b) comprises a nucleotide sequence of SEQ ID NO:44; or (c) comprises a coding portion of a nucleotide sequence of SEQ ID NO:44.