| 发明名称 |
A METHOD OF PREPARING BIOLOGICAL MATERIAL |
| 摘要 |
This invention pertains to a method of preparing biological material from a biological sample selected from the group consisting of blood and sputum samples. The method includes the step of altering at least one constitutive characteristic of the biological sample in the presence of a capturing scaffold by adding a lysis buffer containing a solubilising agent and a detergent to the biological sample, for simultaneously inhibiting coagulation of the biological sample; lysing the biological sample to release the biological material from the biological sample, thus making the biological material available; and capturing at least one fraction of the biological material on the capturing scaffold. |
| 申请公布号 |
US2014370508(A1) |
申请公布日期 |
2014.12.18 |
| 申请号 |
US201214343019 |
申请日期 |
2012.09.06 |
| 申请人 |
Grobler Anne Frederica;Levanets Oksana |
发明人 |
Grobler Anne Frederica;Levanets Oksana |
| 分类号 |
C12N15/10;G01N1/34 |
主分类号 |
C12N15/10 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of preparing biological material from a biological sample selected from the group consisting of blood and sputum samples, the method including the step of altering at least one constitutive characteristic of the biological sample in the presence of a capturing scaffold by adding a lysis buffer containing a solubilising agent and a detergent to the biological sample, for simultaneously inhibiting coagulation of the biological sample; lysing the biological sample to release biological material from the biological sample thus making the biological material available; and capturing at least one fraction of the biological material on the capturing scaffold. |
| 地址 |
Potchefstroom ZA |
