The present invention relates generally to methods for single-cell nucleic acid profiling, and nucleic acids useful in those methods. For example, it concerns using barcode sequences to track individual nucleic acids at single-cell resolution, utilizing template switching and sequencing reactions to generate the nucleic acid profiles. These methods and compositions are also applicable to other starting materials, such as cell and tissue lysates or extracted/purified RNA.
申请公布号
WO2014201273(A1)
申请公布日期
2014.12.18
申请号
WO2014US42159
申请日期
2014.06.12
申请人
THE BROAD INSTITUTE, INC.;PRESIDENT AND FELLOWS OF HARVARD COLLEGE;MIKKELSEN, TARJEI;SOUMILLON, MAGALI