发明名称 |
Phi29 DNA polymerase mutants having increased thermostability and processivity comprising M8R, V51A, M97T, L123S, G197D, K209E, E221K, E239G, Q497P, K512E, E515A, and F526L |
摘要 |
Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template. |
申请公布号 |
EP2813576(A2) |
申请公布日期 |
2014.12.17 |
申请号 |
EP20140165903 |
申请日期 |
2014.04.24 |
申请人 |
THERMO FISHER SCIENTIFIC BALTICS UAB |
发明人 |
POVILAITIS, TADAS;SKIRGAILA, REMIGIJUS |
分类号 |
C12P19/34;C12N9/12;C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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