Rapid production of monoclonal antibodies

摘要

The present invention relates to genetically altered hybridomas, myelomas and B cells. The invention also relates to utilizing genetically altered hybridomas, myelomas and B cells in methods of making monoclonal antibodies. The present invention also provides populations of hybridomas and B cells that can be utilized to make a monoclonal antibody of interest.

主权项

1. A non-human transgenic animal comprising B cells comprising a transgene, wherein the transgene comprises a nucleic acid encoding Igα and Igβ surface-expressed antibody receptor, wherein the nucleic acid is introduced into the germline of the transgenic animal and is incorporated into the animal's genome, and wherein the transgene is expressed in one or more of immature B cells, mature naïve B cells, mature activated B cells, memory B cells, B lineage lymphocytes and plasma cells, wherein expression of the transgene results in an increase in the level of surface-expressed antibody on the surface of one or more immature B cells, mature naïve B cells, mature activated B cells, memory B cells, B lineage lymphocytes and plasma cells relative to non-transgenic cells.