Carbohydrate chip for detection of pathogen Vibrio cholerae and method of preparing the same

摘要

The present invention related to a saccharide-based cholera toxin detection sensor for detection of Vibrio cholerae and its use. More specifically, the present invention relates to a carbohydrate chip for detection of Vibrio cholerae, a method for detecting Vibrio cholerae using the same, and a method for preparing the same, where the carbohydrate chip comprises GM1 pentasaccharide, GM2 tetrasaccharide, asialo GM1 tetrasaccharide, GM3 trisaccharide, galactose-β 1,3-N-acetylgalactosamine, lactose, and sialic acid that are immobilized on the surface of a solid substrate.

主权项

1. A method for detecting Vibrio cholerae by detecting cholera toxin consisting of one A subunit and five B subunits, the method comprising:
applying a sample containing cholera toxin onto a carbohydrate chip, wherein the carbohydrate chip comprises (i) a solid substrate and (ii) GM1 pentasaccharide, GM2 tetrasaccharide, asialo GM1 tetrasaccharide, GM3 trisaccharide, galactose-β 1,3-N-acetylgalactosamine, lactose, and sialic acid immobilized on a surface of the solid substrate, wherein the carbohydrate chip has a visual limit of detection (LOD) of 5 ng/mL for cholera toxin produced by Vibrio cholera; and detecting the cholera toxin by fluorescence image analysis.