Thyroxine immunoassay using fluorescent particles

摘要

Provided are a base plate and a method for thyroxine immunoassay, which are capable of decreasing the signal change rate caused by temperature change in a high-sensitivity immunoassay method for thyroxine. A base plate for thyroxine immunoassay which has a conjugate of thyroxine or a derivative thereof and albumin immobilized thereon, and in which the ratio of the molecule number of thyroxine or a derivative thereof to the molecule number of albumin in the immobilized conjugate, is disclosed.

主权项

1. A base plate having a metal film on the surface for thyroxine immunoassay, comprising a conjugate of thyroxine and bovine serum albumin immobilized on said metal film on the base plate surface, the ratio of the molecule number of thyroxine or a derivative thereof to the molecule number of bovine serum albumin in the immobilized conjugate being from 9.4 to 11.4, and wherein said thyroxine is covalently conjugated with said bovine serum albumin through the amino group or carboxyl group of said thyroxine.