发明名称 |
Tannase, gene encoding same, and process for producing same |
摘要 |
Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived from Aspergillus awamori or Aspergillus niger. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65° C. (pH 5.0, 30 min.). |
申请公布号 |
US8911984(B2) |
申请公布日期 |
2014.12.16 |
申请号 |
US201314088862 |
申请日期 |
2013.11.25 |
申请人 |
Amano Enzyme Inc. |
发明人 |
Nakagawa Megumi;Matsumoto Naoki;Amano Hidoshi;Yamaguchi Shotaro |
分类号 |
C12N1/20;C12N9/14;C12N15/00;C12N9/18;C12P7/42 |
主分类号 |
C12N1/20 |
代理机构 |
Edwards Wildman Palmer LLP |
代理人 |
Edwards Wildman Palmer LLP |
主权项 |
1. A recombinant vector comprising a DNA selected from the group consisting of the following (A) to (D):
(A) a DNA encoding the amino acid sequence set forth in SEQ ID NO: 5; (B) a DNA comprising the base sequence set forth in SEQ ID NO: 4; (C) a DNA having a base sequence that is at least 95% identical to the base sequence set forth in SEQ ID NO: 4 and encoding a protein that has a tannase activity; and (D) a DNA encoding a protein that is at least 95% identical to the amino acid sequence set forth in SEQ ID NO: 5 and that has a tannase activity. |
地址 |
Nagoya-shi JP |