Method for antibiotic susceptibility testing and determining minimum inhibitory concentration of the antibiotic

摘要

A method of antibiotic susceptibility testing is disclosed, and includes the following steps: (A) providing a sample to be tested wherein the sample contains a microbe; (B) adding an antibiotic into the sample, wherein the antibiotic serves to inhibit cell wall synthesis; (C) checking the sample by dielectrophoresis and observing a shape change of the microbe; and (D) determining whether the microbe is susceptible to the antibiotic according to the shape change thereof. The present invention also discloses a method for determining a minimum inhibitory concentration of the antibiotic.

主权项

1. A method of antibiotic susceptibility testing, comprising the following steps:
(A) providing a test sample containing a microbe, wherein the microbe is a Gram-negative rod-shaped bacterium; (B) adding an antibiotic to the test sample, wherein the antibiotic is used to inhibit cell wall synthesis, and wherein the antibiotic is a β-lactam antibiotic; (C) executing dielectrophoresis to the test sample and observing morphologic changes of the microbe in the test sample; and (D) determining whether the microbe is resistant to the antibiotic according to the morphologic changes of the microbe.