Methods of use for an immunoassay detecting fragment Ba

摘要

Methods, compositions and kits for detecting the complement Factor B cleavage product Ba in a biological sample are described. These methods, compositions and kits are useful in convenient, reliable and early diagnosis of or ruling out pre-eclampsia in a pregnant human subject.

主权项

1. A method for ruling out pre-eclampsia in a pregnant human subject, comprising:
contacting a sample of a body fluid obtained at a time period between 8 and 42 weeks of pregnancy from the subject with an antibody to a neo-epitope on the Ba fragment (Ba) of Factor B, said antibody does not substantially recognize Factor B or the Bb fragment (Bb) of Factor B; detecting whether the antibody binds to the neo-epitope on Ba in the sample; and identifying as “ruled-out” those pregnant human subjects for which no binding is detected.