METHOD FOR OBTAINING MAB-LIKE CELLS AND USES THEREOF

摘要

The invention relates to the derivation of mesoangioblast-like (MAB-like) cells from pluripotent cells such as induced pluripotent (IPS) and embryonic stem (ES) cells, to cells obtained thereby and to medical uses of such cells, in particular in the treatment of muscular dystrophies.

主权项

1. Method for obtaining mesoangioblast (MAB)-like mesodermal stem/progenitor cells from pluripotent stem cells comprising the following steps:
(a) dissociating colonies of said cells to a first single cell suspension; (b) seeding the first single cell suspension on a solid support coated with a cell culture substrate with appropriate culture medium and temperature in a low O2 atmosphere to get a first cell culture; (c) dissociating the cell culture to get a second single cell suspension; (d) seeding the second single cell suspension as in step b) to get a second cell culture;wherein steps (c) and (d) are optionally repeated.