| 发明名称 |
Method Of Determining The Viability Of At Least One Cell |
| 摘要 |
This disclosure provides a method of determining the viability of at least one cell via quantification of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), flavin adenine dinucleotide (FAD), and/or collagen. The method includes the steps of contacting the at least one cell with light having a wavelength of from 700 to 900 nm, using two photon excitation, or from 335 to 400 nm, using one photon excitation, to induce an optical response from the NAD(P)H, FAD, and/or collagen and measuring the optical response. The method also includes the step of quantifying one or more of an amount, spatial localization, and/or time-dependent response of the NAD(P)H, FAD, and/or collagen utilizing the optical response. |
| 申请公布号 |
US2014363840(A1) |
申请公布日期 |
2014.12.11 |
| 申请号 |
US201414323790 |
申请日期 |
2014.07.03 |
| 申请人 |
The Regents of the University of Michigan |
发明人 |
Mycek Mary-Ann;Feinberg Stephen;Marcelo Cynthia;Lloyd William;Chen Leng-Chun;Kuo Shiuhang;Kim Hyungjin Myra |
| 分类号 |
G01N33/483 |
主分类号 |
G01N33/483 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of determining the viability of at least one cell via quantification of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), flavin adenine dinucleotide (FAD), and/or collagen, said method comprising the steps of:
A. contacting the at least one cell with light having a wavelength of from 700 to 900 nm, using two photon excitation, or from 335 to 400 nm, using one photon excitation, to induce an optical response from the NAD(P)H, FAD, and/or collagen; B. measuring the optical response; and C. quantifying one or more of an amount, spatial localization, and/or time-dependent response of the NAD(P)H, FAD, and/or collagen utilizing the optical response. |
| 地址 |
Ann Arbor MI US |
