METHOD FOR PREPARING SPHEROIDS OF HUMAN PRIMARY HEPATOCYTES

摘要

The present invention relates to a method for preparing spheroids of human primary hepatocytes. The method comprises culturing of isolated human primary hepatocytes on a polysaccharide scaffold under conditions that allow the formation of hepatocyte spheroids, and subsequently dissolving the polysaccharide scaffold to release the hepatocyte spheroids. The spheroids obtained by the method of the invention are particularly suitable for being transplanted into a subject afflicted with a liver disease.

主权项

1. Isolated hepatocyte spheroid of cultured human primary hepatocytes, wherein said spheroid is free of any artificial scaffold material.