| 摘要 |
The invention provides methods for simultaneously detecting or simultaneously quantifying any combination of thyroxine (T4), triiodothyronine (T3), 3,3′-diiodo-L-thyronine (3,3′-T2), 3-iodothyronamine (T1AM), and, optionally, reverse T3 (rT3) in a sample obtained from a human. The method involves a simple, sensitive, accurate, and specific isotope dilution tandem mass spectrometry method for the simultaneous quantification of any combination of T4, T3, 3,3′-T2, T1AM, and, optionally, rT3 in a sample obtained from a human, e.g., in human plasma or serum samples. This assay is far more sensitive than previously described assays for thyronamines and allows quantitation of T1AM in human plasma or serum, including from healthy controls. |
| 主权项 |
1. A method of simultaneously assaying thyroxine (T4), triiodothyronine (T3), 3,3′-diiodo-L-thyronine (3,3′-T2), and 3-iodothyronamine (T1AM), comprising:
a) providing a test sample, wherein the test sample comprises T4, T3, 3,3′-T2, and T1AM; b) providing a calibration sample, wherein the calibration sample comprises known quantities of reference T4, T3, 3,3′-T2, and T1AM; c) combining the test sample with the calibration sample; d) determining by mass spectrometry the quantity of T4, T3, 3,3′-T2, and T1AM in the test sample and the quantity of the reference T4, T3, 3,3′-T2, and T1AM; and e) calibrating the quantity of the T4, T3, 3,3′-T2, and T1AM in the test sample against the known and determined quantities of the reference T4, T3, 3,3′-T2, and T1AM in the calibration sample. |
