| 摘要 |
FIELD: medicine, pharmaceutics.SUBSTANCE: invention relates to the field of molecular biology and genetic engineering. Claimed are: a method and a set for the detection of poorly represented RNA fractions in a biological sample and for the detection of mycoplasma contamination, for which purpose used are operations of reverse transcription of RNA into cDNA with the application of reverse transcriptase Thermus thermophilus (rTth-pol) under conditions of "hot start", RT inactivation by processing with a chaotropic preparation, detection of cDNA of interest by a polymerase chain reaction (PCR).EFFECT: invention can be used in medicine in testing for the mycoplasma presence.15 cl, 3 tbl, 3 ex |
