CELL COLLECTION METHOD

摘要

To enable collection efficiency of cells to be improved without using a new culture medium or washing liquid for washing purposes. Provided is a method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit, that includes: a supernatant discharge step for transferring a culture supernatant that has been cultured from the culture container to the waste liquid container; a cell collection step for transferring a concentrated suspension of cells from the culture container to the cell collection container; a supernatant returning step for returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells; and re-collection step for transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell culture container.