MULTICOLOUR EXCITATION MODULE FOR A MULTIPHOTON IMAGING SYSTEM, AND ASSOCIATED METHOD AND SYSTEM

摘要

A module is provided for a multi-photon imaging system for simultaneously exciting chromophores of a specimen. A first femtosecond laser source emits a first pulsed excitation beam having a repetition rate 1/T and a wavelengthλ1 exciting a first chromophores, the absorbed photons originating from the first excitation beam. A second femtosecond laser source emits a second pulsed excitation beam with a wavelengthλ2 exciting a second chromophores, the absorbed photons originating from the second beam; the first beam including an“excitation”part exciting the specimen and a“pumping”part acting as a pump beam for exciting the second laser source to synchronize the second laser source with the first laser source. An optical delay line superimposes spatially and temporally the second beam and the excitation part of the first beam to excite at least a third chromophore, the absorbed photons originating from the first and second excitation beams.