METHOD FOR PREPARING HIGH-PURITY VIRION CONCENTRATE

摘要

FIELD: medicine.SUBSTANCE: chicken embryos are infected; a virus-containing allantoic fluid (VAF) is collected, and the VAF is purified by microfiltration. The VAF is purified with the use of polyethylene glycol of the molecular weight of 6000 in the concentration of 1.5% and microfiltered on depth filters having pore diameters of 1.0 mcm and 10.0 mcm. Further, the purified VAF is concentrated by ultrafiltration on membranes with the molecular weight cut 100 kD, and the prepared concentrate is purified in a sucrose gradient by ultracentrifugation.EFFECT: using the method enables the higher quality of virion purification, as well as the higher number of viral antigens recovered from one chicken embryo.1 tbl, 1 dwg, 6 ex