METHOD OF MODELLING ENTEROENTEROANASTOMOSIS IN SMALL LABORATORY ANIMALS

摘要

FIELD: medicine.SUBSTANCE: invention relates to medicine and biology and can be used for studying physiological functions of absorption of various substances and motility in an isolated section of the small intestine by Thiry-Vella in experiment. The abdominal cavity is opened. A purse-string suture in the form of a circle is made on the anterior surface of the stomach. In the centre of the purse-string suture the stomach wall is dissected. A guide catheter with a ball at the end is introduced into the small intestine lumen through the stomach cavity to the place of an anastomosis formation. The small intestine with the mesentery is intersected. After isolation of an isolated section of the small intestine a bolus-tube is fixed on the guide catheter with the ball at the end. An enteroenteroanastomosis between proximal and distal ends of the small intestine on the bolus-tube is formed. Integrity of the intestine and its impermeability are recovered by the application of single-row interrupted serous-muscular-subcutaneous sutures. The guide catheter with the ball at the end and the bolus-tube are removed through an opening in the stomach limited by the purse-string suture. Impermeability and consistency of the enteroenteroanastomosis are checked. Fistula tubes are fixed on the proximal and distal ends of the isolated section of the small intestine.EFFECT: method makes it possible to preserve the normal lumen of the small intestine in the formation of the enteroenteroanastomosis "end-to-end" in small laboratory animals and check consistency and impermeability of the formed anastomosis intraoperationally, as well as to preserve the created model for a long time for studying small intestinal functions due to the application of the purse-string suture on the stomach, application of the guide-catheter with the ball at the end and the bolus-tube, and reliable intestinal sutures.1 ex, 5 dwg