METHOD FOR PATHOGEN ELUTION FROM IMMOBILISED MAGNETIC MATRIX

摘要

FIELD: medicine.SUBSTANCE: method for elution is implemented as follows: 10% tularemic, plague or brucellosis magnetic immune sorbent (MIS) 0.1ml is incubated with microbial suspensions of tularemic, plague or brucellosis agents for 30 min; the supernatant is removed; the MIS is incubated with an eluting solution 0.5 ml for 10 min; the eluate pH is reduced to the physiological value; an indirect hemagglutination reaction or a latex agglutination reaction is carried out.EFFECT: method enables eluting the pathogen from a magnetic immobilised matrix qualitatively to be analysed by serological responses and keeping the antibody functionally active on the magnetic matrix for re-use.3 ex, 1 tbl