| 发明名称 | 用于提高碱性蛋白酶的溶解度的方法 | ||
| 摘要 | 提供在液体洗涤剂中表现出提高的溶解度的碱性蛋白酶。由SEQ ID NO:2所表示的氨基酸序列或与其具有80％或更多序列同一性的氨基酸序列组成的突变碱性蛋白酶，其中选自SEQ ID No:2所表示的氨基酸序列的预定位置或与其相对应的位置处的氨基酸残基的至少一个氨基酸残基被取代。 | ||
| 申请公布号 | CN104204201A | 申请公布日期 | 2014.12.10 |
| 申请号 | CN201380014362.X | 申请日期 | 2013.04.09 |
| 申请人 | 花王株式会社 | 发明人 | 东畑正敏;西村祐美;和田恭尚;佐伯胜久;奥田光美 |
| 分类号 | C12N9/52(2006.01)I;C12N9/54(2006.01)I | 主分类号 | C12N9/52(2006.01)I |
| 代理机构 | 北京尚诚知识产权代理有限公司 11322 | 代理人 | 龙淳 |
| 主权项 | 一种提高碱性蛋白酶在液体洗涤剂中的溶解度的方法，所述方法包括，在由SEQ ID NO:2所表示的氨基酸序列或与其具有80％或更多同一性的氨基酸序列组成的碱性蛋白酶中，将选自SEQ ID No:2所表示的氨基酸序列的表1‑1和表1‑2的列(i)中所述的位置或与其相对应的位置处的氨基酸残基的至少一个氨基酸残基用表1‑1和表1‑2的列(ii)中所述的氨基酸残基取代。[表1‑1]<tables num="0001" id="ctbl0001"><table><tgroup cols="3"><colspec colname="c001" colwidth="10%" /><colspec colname="c002" colwidth="14%" /><colspec colname="c003" colwidth="76%" /><tbody><row><entry morerows="1"> </entry><entry morerows="1">(i)位置</entry><entry morerows="1">(ii)氨基酸残基</entry></row><row><entry morerows="1">(A)</entry><entry morerows="1">405</entry><entry morerows="1">亮氨酸、色氨酸、苯丙氨酸、异亮氨酸、脯氨酸或缬氨酸</entry></row><row><entry morerows="1">(B)</entry><entry morerows="1">81</entry><entry morerows="1">亮氨酸、脯氨酸、酪氨酸、色氨酸、苯丙氨酸、异亮氨酸或谷氨酰胺</entry></row><row><entry morerows="1">(C)</entry><entry morerows="1">40</entry><entry morerows="1">异亮氨酸、苯丙氨酸、亮氨酸、缬氨酸或色氨酸</entry></row><row><entry morerows="1">(D)</entry><entry morerows="1">191</entry><entry morerows="1">亮氨酸、缬氨酸、苯丙氨酸、异亮氨酸或色氨酸</entry></row><row><entry morerows="1">(E)</entry><entry morerows="1">59</entry><entry morerows="1">缬氨酸、异亮氨酸、亮氨酸、苯丙氨酸、甲硫氨酸或色氨酸</entry></row><row><entry morerows="1">(F)</entry><entry morerows="1">11</entry><entry morerows="1">甘氨酸、天冬酰胺或丝氨酸</entry></row><row><entry morerows="1">(G)</entry><entry morerows="1">16</entry><entry morerows="1">异亮氨酸、亮氨酸、缬氨酸或色氨酸</entry></row><row><entry morerows="1">(H)</entry><entry morerows="1">20</entry><entry morerows="1">丙氨酸</entry></row><row><entry morerows="1">(I)</entry><entry morerows="1">22</entry><entry morerows="1">色氨酸</entry></row><row><entry morerows="1">(J)</entry><entry morerows="1">23</entry><entry morerows="1">天冬酰胺</entry></row><row><entry morerows="1">(K)</entry><entry morerows="1">37</entry><entry morerows="1">苏氨酸</entry></row><row><entry morerows="1">(L)</entry><entry morerows="1">41</entry><entry morerows="1">异亮氨酸</entry></row><row><entry morerows="1">(M)</entry><entry morerows="1">52</entry><entry morerows="1">甘氨酸或丝氨酸</entry></row><row><entry morerows="1">(N)</entry><entry morerows="1">53</entry><entry morerows="1">丙氨酸、异亮氨酸或缬氨酸</entry></row><row><entry morerows="1">(O)</entry><entry morerows="1">56</entry><entry morerows="1">缬氨酸</entry></row><row><entry morerows="1">(P)</entry><entry morerows="1">60</entry><entry morerows="1">苯丙氨酸、异亮氨酸、亮氨酸、缬氨酸或色氨酸</entry></row><row><entry morerows="1">(Q)</entry><entry morerows="1">63</entry><entry morerows="1">天冬氨酸或亮氨酸</entry></row><row><entry morerows="1">(R)</entry><entry morerows="1">80</entry><entry morerows="1">丙氨酸或组氨酸</entry></row><row><entry morerows="1">(S)</entry><entry morerows="1">82</entry><entry morerows="1">谷氨酰胺</entry></row><row><entry morerows="1">(T)</entry><entry morerows="1">91</entry><entry morerows="1">半胱氨酸</entry></row><row><entry morerows="1">(U)</entry><entry morerows="1">100</entry><entry morerows="1">苯丙氨酸、异亮氨酸、亮氨酸或色氨酸</entry></row><row><entry morerows="1">(V)</entry><entry morerows="1">101</entry><entry morerows="1">苯丙氨酸、异亮氨酸、亮氨酸、缬氨酸、色氨酸或酪氨酸</entry></row><row><entry morerows="1">(W)</entry><entry morerows="1">109</entry><entry morerows="1">苯丙氨酸、异亮氨酸或亮氨酸</entry></row><row><entry morerows="1">(X)</entry><entry morerows="1">113</entry><entry morerows="1">亮氨酸或色氨酸</entry></row><row><entry morerows="1">(Y)</entry><entry morerows="1">120</entry><entry morerows="1">苯丙氨酸、异亮氨酸、色氨酸或酪氨酸</entry></row><row><entry morerows="1">(Z)</entry><entry morerows="1">135</entry><entry morerows="1">亮氨酸</entry></row><row><entry morerows="1">(AA)</entry><entry morerows="1">140</entry><entry morerows="1">苯丙氨酸、亮氨酸或色氨酸</entry></row><row><entry morerows="1">(AB)</entry><entry morerows="1">151</entry><entry morerows="1">苯丙氨酸</entry></row><row><entry morerows="1">(AC)</entry><entry morerows="1">166</entry><entry morerows="1">苯丙氨酸、异亮氨酸、亮氨酸、缬氨酸或色氨酸</entry></row><row><entry morerows="1">(AD)</entry><entry morerows="1">194</entry><entry morerows="1">酪氨酸</entry></row><row><entry morerows="1">(AE)</entry><entry morerows="1">200</entry><entry morerows="1">色氨酸</entry></row><row><entry morerows="1">(AF)</entry><entry morerows="1">204</entry><entry morerows="1">异亮氨酸、亮氨酸、缬氨酸或甲硫氨酸</entry></row><row><entry morerows="1">(AG)</entry><entry morerows="1">212</entry><entry morerows="1">亮氨酸、缬氨酸或色氨酸</entry></row></tbody></tgroup></table></tables>[表1‑2]<tables num="0002" id="ctbl0002"><table><tgroup cols="3"><colspec colname="c001" colwidth="10%" /><colspec colname="c002" colwidth="14%" /><colspec colname="c003" colwidth="76%" /><tbody><row><entry morerows="1"> </entry><entry morerows="1">(i)位置</entry><entry morerows="1">(ii)氨基酸残基</entry></row><row><entry morerows="1">(AH)</entry><entry morerows="1">233</entry><entry morerows="1">异亮氨酸、亮氨酸或色氨酸</entry></row><row><entry morerows="1">(AI)</entry><entry morerows="1">238</entry><entry morerows="1">亮氨酸</entry></row><row><entry morerows="1">(AJ)</entry><entry morerows="1">243</entry><entry morerows="1">异亮氨酸、亮氨酸或酪氨酸</entry></row><row><entry morerows="1">(AK)</entry><entry morerows="1">246</entry><entry morerows="1">苯丙氨酸、亮氨酸、缬氨酸、色氨酸或酪氨酸</entry></row><row><entry morerows="1">(AL)</entry><entry morerows="1">275</entry><entry morerows="1">苯丙氨酸、亮氨酸或色氨酸</entry></row><row><entry morerows="1">(AM)</entry><entry morerows="1">277</entry><entry morerows="1">苯丙氨酸、异亮氨酸、亮氨酸或缬氨酸</entry></row><row><entry morerows="1">(AN)</entry><entry morerows="1">297</entry><entry morerows="1">苯丙氨酸、亮氨酸或色氨酸</entry></row><row><entry morerows="1">(AO)</entry><entry morerows="1">326</entry><entry morerows="1">色氨酸</entry></row><row><entry morerows="1">(AP)</entry><entry morerows="1">330</entry><entry morerows="1">苯丙氨酸、甲硫氨酸或色氨酸</entry></row><row><entry morerows="1">(AQ)</entry><entry morerows="1">332</entry><entry morerows="1">甘氨酸、苏氨酸或缬氨酸</entry></row><row><entry morerows="1">(AR)</entry><entry morerows="1">334</entry><entry morerows="1">亮氨酸</entry></row><row><entry morerows="1">(AS)</entry><entry morerows="1">342</entry><entry morerows="1">谷氨酸、亮氨酸、苏氨酸或色氨酸</entry></row><row><entry morerows="1">(AT)</entry><entry morerows="1">343</entry><entry morerows="1">苏氨酸</entry></row><row><entry morerows="1">(AU)</entry><entry morerows="1">357</entry><entry morerows="1">亮氨酸</entry></row><row><entry morerows="1">(AV)</entry><entry morerows="1">359</entry><entry morerows="1">苯丙氨酸、异亮氨酸、亮氨酸或甘氨酸</entry></row><row><entry morerows="1">(AW)</entry><entry morerows="1">361</entry><entry morerows="1">异亮氨酸、缬氨酸或色氨酸</entry></row><row><entry morerows="1">(AX)</entry><entry morerows="1">376</entry><entry morerows="1">色氨酸</entry></row><row><entry morerows="1">(AY)</entry><entry morerows="1">378</entry><entry morerows="1">亮氨酸或色氨酸</entry></row><row><entry morerows="1">(AZ)</entry><entry morerows="1">385</entry><entry morerows="1">甲硫氨酸、脯氨酸或酪氨酸</entry></row><row><entry morerows="1">(BA)</entry><entry morerows="1">386</entry><entry morerows="1">丙氨酸、异亮氨酸、亮氨酸或甲硫氨酸</entry></row><row><entry morerows="1">(BB)</entry><entry morerows="1">387</entry><entry morerows="1">苯丙氨酸、甘氨酸、异亮氨酸、亮氨酸、甲硫氨酸、缬氨酸或色氨酸</entry></row><row><entry morerows="1">(BC)</entry><entry morerows="1">390</entry><entry morerows="1">苯丙氨酸、甘氨酸、丝氨酸、苏氨酸或酪氨酸</entry></row><row><entry morerows="1">(BD)</entry><entry morerows="1">393</entry><entry morerows="1">谷氨酰胺</entry></row><row><entry morerows="1">(BE)</entry><entry morerows="1">396</entry><entry morerows="1">甘氨酸</entry></row><row><entry morerows="1">(BF)</entry><entry morerows="1">403</entry><entry morerows="1">赖氨酸或苏氨酸</entry></row><row><entry morerows="1">(BG)</entry><entry morerows="1">406</entry><entry morerows="1">苯丙氨酸、缬氨酸或色氨酸</entry></row><row><entry morerows="1">(BH)</entry><entry morerows="1">407</entry><entry morerows="1">半胱氨酸或甘氨酸</entry></row><row><entry morerows="1">(BI)</entry><entry morerows="1">408</entry><entry morerows="1">异亮氨酸、天冬酰胺、色氨酸或酪氨酸</entry></row><row><entry morerows="1">(BJ)</entry><entry morerows="1">409</entry><entry morerows="1">色氨酸或酪氨酸</entry></row><row><entry morerows="1">(BK)</entry><entry morerows="1">411</entry><entry morerows="1">丙氨酸、亮氨酸、脯氨酸或缬氨酸</entry></row><row><entry morerows="1">(BL)</entry><entry morerows="1">427</entry><entry morerows="1">缬氨酸</entry></row><row><entry morerows="1">(BM)</entry><entry morerows="1">433</entry><entry morerows="1">亮氨酸</entry></row></tbody></tgroup></table></tables> | ||
| 地址 | 日本东京都 | ||