主权项 |
1. A microfluidic device having
a substrate; and a micro-channel structure through which a micro-droplet is transported, the micro-channel structure being formed in the substrate, the micro-channel structure having a first mixing unit, and a second mixing unit connected to a downstream of the first mixing unit, each mixing unit comprising: a first measuring section consisting of a micro-channel having a capacity equal to a volume of a first micro-droplet having a fixed amount, for measuring of a fixed amount of the first micro-droplet; a second measuring section consisting of a micro-channel having a capacity equal to a volume of a second micro-droplet of a fixed amount, for measuring a fixed amount of the second micro-droplet; a merging section for merging the first and the second micro-droplets that have been measured in the first and the second measuring section; a mixing section for mixing the first and the second micro-droplets, the mixing section connected in series to a downstream of the merging section; an exhausting section for exhausting the mixed droplet obtained by mixing the first and the second micro-droplets; a first to a third inlet ports and a first to a third outlet ports; a first micro-channel for connecting the first inlet port and the first outlet port; a second micro-channel having the merging section, the mixing section, and the exhausting section, the second micro-channel connecting the second inlet port and the second outlet port; a third micro-channel for connecting the third inlet port and the third outlet port; an end of the first measuring section being connected to the first micro-channel, another end of the first measuring section having an opening in the merging section provided in the second micro-channel, an end of the second measuring section being connected to the third micro-channel, another end of the second measuring section having an opening in the merging section provided in the second micro-channel, the second outlet port being connected to the exhausting section, one of the outlet ports in the first to the third outlet ports of the first mixing unit being connected to the first or the third inlet port of the second mixing unit, wherein the second outlet port of the first mixing unit is connected to and in fluid communication with the first inlet port of the second mixing unit such that liquid exiting the second outlet port enters the first inlet port of the second mixing unit. |