Method for screening ameliorants of dry skin caused by atopic dermatitis using bleomycin hydrolase activity as indicator

摘要

The present invention provides a method for screening and evaluating ameliorants of dry skin caused by atopic dermatitis, comprising: evaluating a candidate drug as being an ameliorant of dry skin caused by atopic dermatitis in the case the candidate drug significantly increases expression and/or activity of bleomycin hydrolase in comparison with a control drug.

主权项

1. A method for screening and evaluating ameliorants of dry skin caused by atopic dermatitis in a human subject, comprising: evaluating a candidate drug as being an ameliorant of dry skin caused by atopic dermatitis in the case the candidate drug significantly increases expression and/or activity of bleomycin hydrolase in comparison with a control drug in human cells, wherein expression and/or activity of bleomycin hydrolase is judged to have increased significantly in the case the transcription activity of a gene that encodes bleomycin hydrolase has increased significantly in comparison with that of a control and wherein the transcription activity is judged to have increased significantly in the case binding activity of transcription factors IRF-1, IRF-2, MZF-1, Sp-1 and/or GATA-1 to the transcription regulatory region of a gene that encodes bleomycin hydrolase has increased significantly in comparison with that of a control.