Method for identifying agents that inhibit cell migration, promote cell adhesion and prevent metastasis

摘要

Disclosed are methods for identification of agents that modulate cell attachment, cell migration and cell viability. Cancer and primary cells adhered to a matrix are treated with agent(s) that modulate ActRII signaling and cell adhesion. Agents are tested that modulate cell adhesion, detachment, invasion and viability. Agents that modulate the expression, phosphorylation, function and translocation of ActRII signaling pathway members also can predict agents that modulate cell adhesion, detachment, invasion and viability. The methods have utility in tissue engineering as well as identifying agents that prevent cancer cell metastasis, wound dehiscence, aortic dissection and aid retina attachment and skin replacement and fertility. Examples of tissue engineering include techniques that allow for the generation of biological substitutes that restore, maintain, or improve tissue function or a whole organ.

主权项

1. A composition to induce cell detachment, wherein the activin receptor II signaling suppressor includes the siRNA having at least one of the sequences SEQ ID NO 12, SEQ ID NO 13, SEQ ID NO 14, and SEQ ID NO 15 comprising a concentration of an siRNA form of activin receptor II signaling suppressor sufficient to induce cell detachment.