Single molecule loading methods and compositions

摘要

Methods for non-random loading of single analyte molecules into array structures are provided. The methods allow for distribution of a population of target molecules into a plurality of size confined regions such as wells. Sizing moieties are linked to individual target molecules. The sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, such that only a selected number of sizing moieties will fit into the size confined regions. The confined regions and the sizing moieties or target molecules comprise a selected charge that allow for controlling the loading of the sizing moities.

主权项

1. A method of distributing a population of target molecules into a plurality of size confined reaction or observation regions, the method comprising:
providing a structure comprising the plurality of size confined reaction or observation regions, wherein such regions comprise wells;
providing the population of target molecules to be distributed;linking at least one nucleic acid sizing moiety to individual target molecules, wherein the sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, such that only a single sizing moiety will fit into each size confined region, thereby preventing the entry of an additional sizing moiety; and,loading the target molecules into the regions such that the size of the confined regions results in a single target molecule distributed into each region, thereby distributing the population of target molecules into the plurality of size confined regions.