Increasing NADPH-dependent products

摘要

A method of increasing cellular NADPH levels by expressing one or more genes that encode an enzyme that causes the production of NADPH. The system is combined with other enzymes that require NADPH, thus improving the overall yield of the desired product.

主权项

1. An engineered Escherichia coli comprising:
a. a deleted gapA (ΔgapA) gene; b. an overexpressed DNA encoding gapC (an NADP-dependent D-glyceraldehyde-3-phosphate dehydrogenase (NADP-GAPDH)); and c. an overexpressed DNA encoding:
i. a β-ketothiolase, an acetoacetyl-CoA reductase and a polyhydroxybutyric acid (PHB) synthase, wherein said cell produce 5-10 fold more PHB than a control cell lacking a) and b); orii. a cyclohexanone monooxygenase, wherein said cell produces about 1.7 fold more caprolactone than a control cell lacking a) and b), oriii. a phytoene synthase, a geranylgeranyl pyrophosphate (GGDP) synthase, and phytoene desaturase, wherein said cell produces about 2.5 fold more lycopene than a control cell lacking a) and b).