Method and kit for performing profiling of endarterectomy patients

摘要

A method of profiling endarterectomy patients for determining one or more post-operative risks includes steps comprising:
(a) obtaining a set of parameters which describe the patient, the set of parameters including at least one of: age of patient, E; smoking habits of patient, S; serum cholesterol concentration of patient, C; diabetes status of patient, D; blood pressure of patient, P;(b) obtaining one or more samples of plaque tissue from endarterectomy treatment of the patient;(c) isolating genetic material from the one or more samples of plaque tissue;(d) determining genetic expression activities of a plurality of genes identified in the genetic material; and(e) computing from the set of parameters in combination with the genetic expression activities one or more post-operative risks indicative of potential events to which the patient is susceptible as a consequence of the endarterectomy treatment.

主权项

1. A method of profiling endarterectomy patients for determining one or more post-operative risks, characterized in that said method includes:
(a) obtaining a set of parameters which describe the patient, said set of parameters including at least one of: age of patient, E; smoking habits of patient, S; serum cholesterol concentration of patient, C; diabetes status of patient, D; blood pressure of patient, P; (b) obtaining one or more samples of plaque tissue from endarterectomy treatment of said patient; (c) isolating mRNA from said one or more samples of plaque tissue; (d) determining genetic expression activities of a plurality of genes identified in said genetic material; and (e) computing from said set of parameters in combination with said genetic expression activities one or more post-operative risks indicative of potential events to which the patient is susceptible as a consequence of said endarterectomy treatment; and in step (e), a blinded cross-validation iterative computation is performed for computing said one or more risks.