发明名称 |
Fremgangsmåde til konstruktion af bæremiddel til gentransport |
摘要 |
An object of the present invention is to provide a method of efficiently constructing a gene delivery carrier having a favorable activity and expression efficiency of a protein expressed by a gene introduced by transformation. Moreover, an object of the present invention is to provide a pharmaceutical composition comprising a gene delivery carrier constructed by the construction method and a therapeutic agent for solid tumor comprising the resistant bacterium. The objects of the present invention have been attained by a gene delivery carrier consisting of an anaerobic microorganism capable of growing in a tumor tissue in an anaerobic environment and capable of expressing (A) a protein having an antitumor activity or (B) a protein having an activity of converting an antitumor substance precursor to an antitumor substance, wherein DNA encoding the protein of interest is allowed to further comprise, on the 5'-terminal side thereof, a DNA fragment comprising a nucleotide sequence encoding a peptide consisting of an amino acid sequence from the 1st to at least 4th amino acids at the N-terminus of a histone-like DNA-binding protein. |
申请公布号 |
DK2468861(T3) |
申请公布日期 |
2014.12.08 |
申请号 |
DK20120151228T |
申请日期 |
2007.05.24 |
申请人 |
ANAEROPHARMA SCIENCE INC. |
发明人 |
SHIMATANI, YUKO;HAMAJI, YOSHINORI;SHIMIZU, HITOMI;AMANO, JUN;TANIGUCHI, SHUN'ICHIRO;FUJIMORI, MINORU |
分类号 |
C12N15/09;A61K31/513;A61K35/74;A61K48/00;A61P35/00;C12N1/21;C12N9/78 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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