Methods for Laser Speckle Contrast Imaging of Blood Perfusion

摘要

A method for imaging blood flow through target tissue is disclosed. An example method may include (a) directing a light beam at a blood-perfused target tissue, (b) reflecting the directed light beam off of static target tissue and flowing cells, (c) capturing a plurality of digital images of interference patterns of the reflected light in a plurality of successive frames, (d) measuring a light intensity of at least one pixel of each digital image, where the at least one pixel corresponds to an identical pair of coordinates in each successive frame, (e) comparing the measured light intensity of the at least one pixel of each digital image to the measured light intensity of the at least one pixel of an adjacent frame at the identical pair of coordinates and (f) determining a compiled light intensity for the at least one pixel for an aggregate motion contrast.

主权项

1. A method, the method comprising:
directing a light beam at a blood-perfused target tissue; reflecting the directed light beam off of static target tissue and flowing cells; capturing a plurality of digital images of interference patterns of the reflected light in a plurality of successive frames; measuring, via a processor, a light intensity of at least one pixel of each digital image, wherein the at least one pixel corresponds to an identical pair of coordinates in each successive frame; comparing the measured light intensity of the at least one pixel of each digital image to the measured light intensity of the at least one pixel of an adjacent frame at the identical pair of coordinates thereby enhancing a light saturation profile for the at least one pixel of each frame; and determining, via the processor, a compiled light intensity for the at least one pixel for an aggregate motion contrast image based upon the enhanced light saturation profile of the at least one pixel for each frame.