HIGH-PURITY STEVIOL GLYCOSIDES

摘要

Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and M are described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2 and reb M2 are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

主权项

1. A method for producing highly purified target steviol glycosides, comprising the steps of:
a. providing a starting composition comprising an organic compound with at least one carbon atom; b. providing a microorganism containing at least one enzyme selected from steviol biosynthesis enzymes, UDP-glycosyltransferases, and optionally UDP-glucose recycling enzymes; c. contacting the microorganism with a medium containing the starting composition to produce a medium comprising at least one target steviol glycoside.