SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS

摘要

A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.

主权项

1. A system for isolating cells in at least one of single-cell format and single-cluster format, comprising:
a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well of the set of wells including:
a base surface,an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, anda set of channels that fluidly couple each well to at least one adjacent well in the set of wells;wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells, wherein the fluid delivery module includes an absorbant layer proximal the broad surface of the substrate having an opening surrounding the array.