INFLUENZA VIRUS VACCINES AND USES THEREOF

摘要

The present disclosure provides influenza hemagglutinin stem domain polypeptides comprising (a) an influenza hemagglutinin HA1 domain that comprises an HA1 N-terminal stem segment, covalently linked by a linking sequence of 0-50 amino acid residues to an HA1 C-terminal stem segment, and (b) an influenza hemagglutinin HA2 domain, wherein on or more amino acids in the HA2 domain have been mutated. Also provided are nucleic acids encoding the polypeptides, compositions comprising the polypeptides and/or nucleic acid molecules, as well as methods of their use, in particular in the detection, prevention and/or treatment of influenza.

主权项

1. An influenza hemagglutinin stem domain polypeptide comprising:
(a) an influenza hemagglutinin HA1 domain that comprises an HA1 N-terminal stem segment, covalently linked by a linking sequence of 0-50 amino acid residues to an HA1 C-terminal stem segment, and (b) an influenza hemagglutinin HA2 domain, wherein the hemagglutinin stem domain polypeptide is resistant to protease cleavage at the junction between HA1 and HA2, wherein one or more amino acids in the amino acid sequence connecting the A helix and the helix CD of HA2 has or have been mutated as compared to a wild-type influenza HA2 domain, and wherein the HA1 domain and HA2 domain are derived from an influenza A virus subtype selected from the group consisting of H1, H5, and H3.