METHOD AND APPARATUS FOR MEASURING PHOSPHORYLATION KINETICS ON LARGE ARRAYS

摘要

The disclosure provides for methods and apparatuses relating to technology for monitoring chemical and/or biological reactions. Some methods provided herein relate to utilization of NAPPA technology to create large protein arrays suitable for use in combination with various ISFET arrays to enable massive parallel assays of kinase activity and inhibition. Some devices provided herein relate to CMOS chips which utilize the NAPPA array technology to build protein inventories of interest upon an ISFET architecture. Further devices provided herein are capable, inter alia, of processing the arrays created by the combination of NAPPA technology and ISFET architecture.

主权项

1. A device for detecting phosphorylation of an array of proteins, comprising:
an array of sensors, each sensor comprising one ion-sensitive field effect transistor (ISFET); a layer of first material disposed over the array of sensors and having formed therein a plurality of microwells, each microwell disposed over at least one of the ISFETs; and wherein each microwell comprises a polypeptide comprising a test amino acid sequence and an affinity tag, and a binding agent that recognizes the affinity tag attached to a surface of the microwell.