RNA-Guided Transcriptional Regulation

摘要

Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

主权项

1. A method of altering a target nucleic acid in a cell comprising
providing to the cell two or more RNAs with each RNA being complementary to an adjacent site in the target nucleic acid, providing to the cell a Cas9 protein nickase and being guided by the two or more RNAs, and wherein the two or more RNAs and the Cas9 protein nickase co-localize to the DNA target nucleic acid and nick the target nucleic acid resulting in two or more adjacent nicks.