Compositions and Methods for the Detection of DNA Cleavage Complexes

摘要

Compositions, methods, and kits for identifying protein-nucleic acid complexes, particularly DNA topoisomerase II-DNA complexes, are disclosed.

主权项

1. A method for identifying sequences present in protein-nucleic acid complexes, comprising:
a) cleaving said protein-nucleic acid complexes by contacting the protein-nucleic acid complexes with a phosphatase; b) contacting the free nucleic acid molecules with a polymerase and a polynucleotide kinase, c) ligating adaptors to the free nucleic acid molecules; d) amplifying the free nucleic acid molecules of step c); and e) identifying the sequence of the amplified nucleic acid molecules, thereby identifying the sequences present in said protein-nucleic acid complexes.