IN VIVO LABEL-FREE HISTOLOGY BY PHOTOACOUSTIC MICROSCOPY OF CELL NUCLEI

摘要

The present disclosure is directed to methods of non-invasively imaging cell nuclei. More particularly, the present disclosure is directed to methods of imaging cell nuclei in vivo using ultraviolet photoacoustic microscopy (UV-PAM), in which ultraviolet light is used to excite unlabeled DNA and RNA in cell nuclei to produce photoacoustic waves.

主权项

1. An ultraviolet photoacoustic imaging apparatus comprising:
a focusing assembly configured to receive ultraviolet light and to focus the ultraviolet light on a biological sample; at least one transducer configured to receive photoacoustic waves emitted by the biological sample in response to the ultraviolet light, said at least one transducer positioned such that said at least one transducer and said focusing assembly are coaxial; and a processor configured to record and process the received photoacoustic waves.