NUCLEIC ACID ENRICHMENT USING CAS9

摘要

A method of enriching for a fragment of a genome, as well as corresponding compositions and kits, are provided. In certain embodiments, the method comprises: (a) contacting a sample comprising fragmented DNA with a Cas9-gRNA complex comprising mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in the DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented DNA; and (b) isolating the complex. In addition, other methods and compositions for Cas9/CRISPR-mediated nucleic acid manipulation are also provided.

主权项

1. A method comprising:
(a) contacting a sample comprising fragmented genomic DNA with a Cas9-gRNA complex comprising a mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in said genomic DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented genomic DNA; and (b) isolating said Cas9-fragment complex.