Apparatus and methodology for flow fluorescence microscopic imaging

摘要

The present invention is to provide a flow fluorescence microscopic imaging apparatus. More particularly, it relates to a fluorescence microscopic imaging apparatus which combines the fluorescence microscopy excited by light sheet and flow cytometry. The present invention provides a new methodology in the field of flow cytometry based on the flow fluorescence microscopic imaging device, which is able to solve the problem of small depth-of-field (DOF) and the motion blur when the flow objects are imaged.

主权项

1. A flow fluorescence microscopy imaging apparatus comprising a light sheet generation unit, a liquid sample delivery unit, and a fluorescence microscopic imaging unit, wherein said light sheet generation unit comprises a light source, said light source comprising a laser light source capable of forming a laser light sheet; flow direction of the liquid sample delivery unit and optical axis of the fluorescence imaging unit are configured to be parallel to each other and perpendicular to the optical axis of the light sheet generation unit.