Cell Surface Display, Screening and Production of Proteins of Interest

摘要

Aspects of the invention provide compositions and methods for displaying engineered polypeptides on a cell surface. According to aspects of the invention, immobilized polypeptides can be screened to identify one or more variants having one or more functional or structural properties of interest. Aspects of the invention provide composition and methods for producing engineered protein or protein variants having a functional or a structural property of interest.

主权项

1. A protein screening method comprising:
a. expressing an engineered protein comprising a modification motif in a host cell having a cell surface comprising a first binding partner,wherein a second binding partner is coupled to the expressed engineered protein, andwherein the expressed engineered protein is secreted and displayed on the cell surface via binding of the second binding partner to the first binding partner; and,
b. evaluating a property of the engineered protein displayed on the cell surface.