| 发明名称 |
METHOD FOR TRANSCRIPTIONAL AMPLIFICATION OF NUCLEIC ACIDS COMBINING STEPS OF DIFFERENT TEMPERATURES |
| 摘要 |
A method of transcriptional amplification includes: (a) obtaining a mixture by combining (i) a biological sample comprising nucleic acids, (ii) amplification primers, (iii) amplification reagents including enzymes required for amplification, and (iv) at least one polyol capable of stabilizing the enzymes required for amplification; (b) denaturing the nucleic acids by heating the mixture at a temperature above 41° C.; and (c) transcriptionally amplifying at least one target nucleic acid at a temperature above 41° C. when present in the mixture. |
| 申请公布号 |
US2014356870(A1) |
申请公布日期 |
2014.12.04 |
| 申请号 |
US201214363214 |
申请日期 |
2012.12.14 |
| 申请人 |
BIOMERIEUX |
发明人 |
Laayoun Ali;Laurent Alain;Mesta Laurent |
| 分类号 |
C12P19/34;C12Q1/68 |
主分类号 |
C12P19/34 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of transcriptional amplification, comprising:
a) obtaining a mixture by combining (i) a biological sample comprising nucleic acids, (ii) amplification primers, (iii) amplification reagents including enzymes required for amplification, and (iv) at least one polyol capable of stabilizing the enzymes required for amplification; b) denaturing the nucleic acids by heating the mixture at a temperature above 44° C.; and c) transcriptionally amplifying at least one target nucleic acid at a temperature above 44° C. when present in the mixture. |
| 地址 |
Marcy-L'Etolie FR |
