The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.
申请公布号
EP2806754(A1)
申请公布日期
2014.12.03
申请号
EP20130700929
申请日期
2013.01.23
申请人
DSM IP ASSETS B.V.
发明人
KUMAR, MANOJ;VAN DEN BERG, MARCO ALEXANDER;BOER, VIKTOR MARIUS;LAWRENCE, ADAM G.;LEHMANN, MARTIN;MEIJRINK, BERNARD;SAGT, CORNELIS MARIA JACOBUS;TRUEHART, JOSHUA;WU, LIANG;ZWARTJENS, PRISCILLA