DNA LOADED SUPPORTED GOLD NANOPARTICLES, PROCESS FOR THE PREPARATION AND USE THEREOF
摘要
<p>The present invention relates to DNA loaded gold nanoparticles embedded in sharp carbonaceous carriers useful for higher DNA delivery efficiently into plants. These nanogold embedded carbon matrices are prepared by heat treatment of biogenic intracellular gold nanoparticles. The DNA delivery efficiency is tested on model plants. These materials reveal good dispersion of the transport material, producing a greater number of GUS foci per unit area. The added advantages of the composite carrier are the lower plasmid and gold requirements. Plant cell damage with the prepared carbon supported particles is very minimal and can be gauged from the increased plant regeneration and transformation efficiency compared to that of the commercial micrometer sized gold particles. This can be attributed to the sharp edges that the carbon supports possess, which lead to better piercing capabilities with minimum damage.</p>
申请公布号
EP2462227(B1)
申请公布日期
2014.12.03
申请号
EP20100760106
申请日期
2010.08.03
申请人
COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
发明人
PRASAD, L. V., BHAGAVATULA;PERIYASAMY, SHANMUGHAM, VIJAYKUMAR;OTHALATHARA, USHARRAJ, ABHILASH;BASHIR, MOHAMMAD, KHAN