发明名称 |
Method for the cultivation of microorganisms of the genus thraustochytriales by using an optimized low salt medium |
摘要 |
The invention relates to an optimized method for cultivating microorganisms of the genus Thraustochytriales, according to which the microorganisms are cultivated in a low salt medium without adding sodium salts and chloride salts, the total salt content being less than 3.5 g/L (corresponding to less than 10 percent of sea water salt content), whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention especially relates to novel optimized media having a substantially reduced total salt content, above all a particularly reduced NaCl content. The production of PUFAs can be substantially improved and significantly simplified by using a novel combination of different salts as a media composition in which the overall weight ratios of ions do not exceed 1.75 g/L. Furthermore, said medium preferably contains no added sodium salt and chloride salt at all, which helps prevent environmental damages caused by wastewaters containing salt. |
申请公布号 |
US8900831(B2) |
申请公布日期 |
2014.12.02 |
申请号 |
US200410578968 |
申请日期 |
2004.11.10 |
申请人 |
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发明人 |
Rüsing Matthias;Luy Markus |
分类号 |
C12P7/64;C12N1/00 |
主分类号 |
C12P7/64 |
代理机构 |
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代理人 |
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主权项 |
1. A method for cultivating microorganisms of the order Thraustochytriales, wherein the microorganisms are cultivated in a fermentation medium characterized in that the sum of the weight fractions of sodium and chloride ions is less than 1.75 g/L and that the total salt content of the fermentation medium is less than 3.5 g/L, and wherein during cultivation the microorganisms bring forth a production of more than 10 wt % DHA per dry biomass; and
characterized in that the microorganism is selected from the group consisting of Ulkenia sp. SAM 2179 and Schizochytrium sp. SR 21. |
地址 |
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