Methods and solutions for tissue preservation

摘要

Described herein are compositions and methods particularly useful in the medical arts. The compositions and methods may be used in connection with the preservation of a portion of a mammal, for example, tissues, organs, appendages, limbs, extremities, stem cells, myocytes, bone marrow, skeletal muscle as well as an array of other medical procedures, such as cardiac surgery, transplantation and/or preservation. In various embodiments, the inventive composition may be hyperoxygenated and be formulated to resemble the biochemistries of natural intracellular fluids. The inventive composition includes active ingredients to reduce ischemic, hypothermic and reperfusion injury during transplantation, thereby resulting in improved post-transplant graft function and quality, when used in connection with organ transplantation and storage procedures, for example cardiac transplantation.

主权项

1. A method of preserving a portion of a mammal comprising:
placing the portion of the mammal in contact with a preservation solution during storage, wherein the preservation solution comprises
a quantity of water,an energy source capable of being metabolized through the tricarboxylic acid cycle comprising a quantity of glucose, a quantity of glutamate, a quantity of glutamine and a quantity of glycine,reductants comprising glutathione and allopurinol,electrolytes comprising sodium chloride, potassium chloride, magnesium sulfate, potassium phosphate (KH2PO4/K2HPO4),a quantity of dissolved oxygen, wherein the quantity of dissolved oxygen produces an oxygen tension of 490 mmHg to 590 mmHg, wherein the osmolarity of the solution is from 325 mOsm to 335 mOsm, the pH is from 6.5 to 7.0 and whereby the portion of the mammal is preserved during storage.