Pipetting device, modular pipetting unit, pipetting system and method for pipetting of fluid samples

摘要

A pipetting device having a modular pipetting unit including a pipetting tip for pipetting of fluid samples and a pump conduit for transferring a negative or positive pressure to the pipetting tip is disclosed. The pipetting tip and a portion of the pump conduit adjoining the pipetting tip mutually define a fluid sample conduit for receiving the fluid samples. The modular pipetting unit is detachably attached to an automated positioning device for positioning the modular pipetting unit. A system and method for pipetting of fluid samples using such a pipetting device are also disclosed wherein pipetting of the fluid samples is performed in such a manner that each pipetted fluid sample volume is smaller than a volume of the fluid sample conduit.

主权项

1. A method for pipetting of fluid samples, said method comprising:
providing a pipetting device comprising at least one pipetting unit provided with a pipetting tip for pipetting of a fluid sample and a pump conduit for transferring a negative or positive pressure to said pipetting tip, said pipetting unit comprising a hollow housing comprising an inner wall surrounding a cavity and a plurality of guiding faces arranged along the inner wall guiding the pump conduit, said pump conduit comprising a first vertical conduit portion extending in the direction of gravity, a second horizontal conduit portion, and a first curved conduit portion connecting said first vertical conduit portion to said second horizontal conduit portion, said first vertical conduit portion being longer than the second horizontal conduit portion, wherein said pipetting tip and at least a linearly extending portion of said first vertical conduit portion of said pump conduit adjoining the pipetting tip mutually define a fluid sample conduit for receiving said fluid sample; controlling the pipetting of said fluid sample such that said fluid sample is aspirated into said first vertical conduit portion but not beyond and into said first curved conduit portion; and pipetting said fluid sample into but not beyond said linearly extending portion such that a pipetted fluid sample volume is smaller than a volume of said fluid sample conduit of said at least one pipetting unit.