Oxygen-regulated microorganisms

摘要

The present invention relates to novel strains of microorganisms with oxygen-regulated metabolism. The microorganisms have higher growth rates and are more efficient than parental strains. The microorganisms may be used to produce a variety of products of interests, such as recombinant proteins, nucleic acids, such as DNA, amino acids, and chemicals.

主权项

1. An E. coli comprising an oxygen-regulated promoter selected from the group consisting of a cytochrome o oxidase (cyo) promoter and a superoxide dismutase (sodA) promoter operatively linked to a sequence selected from the group consisting of a sequence comprising the cscA gene of the E. coli O157.H7 chromosomally-encoded sucrose catabolism (csc) module and a sequence comprising the ptsH, ptsI and carbohydrate repression resistant (crr) genes of the E. coli phosphoenol pyruvate-dependent phosphotransferase system (pts) wherein metabolic flux from a carbon source in the E. coli is decreased in response to a reduction in oxygen levels and wherein the E. coli is sucrose catabolizing (Suc+) when said oxygen-regulated promoter is operatively linked to said sequence comprising the cscA gene.