Method for the preparation of 1,3-propanediol from sucrose

摘要

A microorganism genetically modified for the bioproduction of 1,3-propanediol from sucrose, wherein the microorganism includes:
a two-step metabolic pathway for the production of 1,3-propanediol, including a first step of decarboxylation of 4-hydroxy-2-ketobutyrate with an enzyme having a 2-keto acid decarboxylase activity, and a second step of reduction of the obtained 3-hydroxypropionaldehyde with an enzyme having hydroxy aldehyde reductase activity, andgenes enabling the microorganism to utilize sucrose as sole carbon source.;A method for the biological preparation of 1,3-propanediol by fermentation, including cultivating said microorganism genetically modified, wherein the culture is performed in an appropriate medium including a source of sucrose, and recovering the 1,3-propanediol being produced.

主权项

1. A method for the fermentative production of 1,3-propanediol from sucrose, comprising the steps of:
culturing a microorganism from the Enterobacteriaceae family genetically modified for the bioproduction of 1,3-propanediol from sucrose on an appropriate culture medium comprising sucrose, wherein the microorganism comprises: (i) an enzyme having a 2-keto acid decarboxylase activity for decarboxylation of 4-hydroxy-2-ketobutyrate encoded by the kivD gene of Lactococcus lactis, and (ii) an enzyme having hydroxy aldehyde reductase activity encoded by the gene yqhD of Escherichia coli for reduction of 3-hydroxypropionaldehyde obtained from decarboxylation of 4-hydroxy-2-ketobutyrate by the 2-keto acid decarboxylase, and (iii) functional genes coding for a non-PTS sucrose utilization system, enabling the microorganism to utilize sucrose as sole carbon source involving the genes cscBKA from E. coli, and wherein the production of 4-hydroxy-2-ketobutyrate from sucrose is improved in said microorganism by overexpressing a gene encoding homoserine transaminase or homoserine oxidase,and
recovering 1,3-propanediol from the culture medium.